Effect of acute alcohol intoxication on the total amount and phosphorylation of eIF4G in skeletal muscle from young and mature rats. Groups are the same as described in Figure 1. Gastrocnemius was collected 2.5 h after administration of alcohol or saline (control). Inset at top: representative Western blots of phosphorylated and total eIF4G. Top and middle graphs: densitometric analysis of immunoblots of Ser1108-phosphorylated eIF4G and total eIF4G, respectively, in muscle. Bottom graph: ratio of phosphorylated (P) to total eIF4G in muscle. Values (means ± SEM) are expressed relative to the young saline-treated control group. Sample size was 10, 10, 6, 7, 9, and 9 for the six groups, respectively. Values with different letters are significantly different from each other, P < 0.05. Values which share a common letter are not statistically different.