Figure 5

Effect of acute alcohol intoxication on the binding of mTOR with raptor in skeletal muscle from young and mature rats. Groups are the same as described in Figure 1. Gastrocnemius was collected 2.5 h after administration of alcohol or saline (control). Inset at top: raptor was immunoprecipitated (IP) and the amount of mTOR and raptor determined by immunoblotting (IB). Bottom graph: densitometric analysis of immunoblots of mTOR bound to immunoprecipitated raptor in muscle. There was no difference among the various groups for the amount of immunoprecipitated raptor (mean data not shown). Values (means ± SEM) are expressed relative to the young saline-treated control group. Sample size was 10, 10, 6, 7, 9, and 9 for the six groups, respectively. Values with different letters are significantly different from each other, P < 0.05. Values which share a common letter are not statistically different.