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Table 4 Effect of acute alcohol intoxication on plasma hormone and metabolic substrate concentrations in young and mature adult male F344 rats

From: Alcohol-induced decrease in muscle protein synthesis associated with increased binding of mTOR and raptor: Comparable effects in young and mature rats

 

Young

Mature – Low Dose EtOH*

Mature – High Dose EtOH

 

Control

Alcohol

Control

Alcohol

Control

Alcohol

Insulin, ng/ml

1.09 ± .09a

0.84 ± .13a

2.13 ± .25b

2.03 ± .21b

2.12 ± .33b

2.09 ± .24b

Testosterone, ng/ml

2.8 ± 0.2a

1.6 ± 0.2b

2.7 ± 0.2a

1.3 ± 0.2b

2.5 ± 0.3a

1.5 ± 0.3b

Estradiol, pg/ml

2.7 ± 0.5

2.1 ± 0.5

3.5 ± 0.4

2.9 ± 0.4

3.6 ± 1.2

2.7 ± 0.4

Glucose, mmol/L

7.0 ± 0.5a

11.2 ± 1.1b

8.4 ± 0.5a

8.7 ± 0.4a

8.5 ± 0.5a

10.8 ± 0.8ab

HOMA

8.3 ± 0.8a

10.3 ± 1.3a

19.6 ± 3.3b

21.6 ± 3.1b

19.6 ± 3.4b

23.7 ± 4.3b

Leucine, μmol/L

95 ± 6a

137 ± 11b

97 ± 4a

107 ± 10a

95 ± 6a

141 ± 12b

Isoleucine, μmol/L

81 ± 10

104 ± 11

85 ± 7

91 ± 11

86 ± 8

116 ± 9

Valine, μmol/L

138 ± 12

166 ± 21

127 ± 15

134 ± 12

133 ± 14

155 ± 12

  1. Values are means ± SEM where the sample size was 10, 10, 6, 7, 9, and 9 for the six groups, respectively. *Mature rats were administered either "low" dose alcohol (75 mmol/kg) or "high" dose alcohol (90 mmol/kg); young rats were injected with 75 mmol/kg. Blood was obtained from overnight fasted rats immediately prior to determination of muscle protein synthesis (e.g., time 0). Glucose and insulin concentrations were determined at time 0 and 10 min after injection of phenylalanine, and values averaged because there was no statistical difference between time points (data not shown). Values with different letters for a particular hormone are significantly different from each other, P < 0.05. Values with the same letter are not significantly different. HOMA = homeostasis model assessment for insulin resistance which is calculated as reported by Matthews et al [25].