Skip to main content

Table 4 Effect of acute alcohol intoxication on plasma hormone and metabolic substrate concentrations in young and mature adult male F344 rats

From: Alcohol-induced decrease in muscle protein synthesis associated with increased binding of mTOR and raptor: Comparable effects in young and mature rats

  Young Mature – Low Dose EtOH* Mature – High Dose EtOH
  Control Alcohol Control Alcohol Control Alcohol
Insulin, ng/ml 1.09 ± .09a 0.84 ± .13a 2.13 ± .25b 2.03 ± .21b 2.12 ± .33b 2.09 ± .24b
Testosterone, ng/ml 2.8 ± 0.2a 1.6 ± 0.2b 2.7 ± 0.2a 1.3 ± 0.2b 2.5 ± 0.3a 1.5 ± 0.3b
Estradiol, pg/ml 2.7 ± 0.5 2.1 ± 0.5 3.5 ± 0.4 2.9 ± 0.4 3.6 ± 1.2 2.7 ± 0.4
Glucose, mmol/L 7.0 ± 0.5a 11.2 ± 1.1b 8.4 ± 0.5a 8.7 ± 0.4a 8.5 ± 0.5a 10.8 ± 0.8ab
HOMA 8.3 ± 0.8a 10.3 ± 1.3a 19.6 ± 3.3b 21.6 ± 3.1b 19.6 ± 3.4b 23.7 ± 4.3b
Leucine, μmol/L 95 ± 6a 137 ± 11b 97 ± 4a 107 ± 10a 95 ± 6a 141 ± 12b
Isoleucine, μmol/L 81 ± 10 104 ± 11 85 ± 7 91 ± 11 86 ± 8 116 ± 9
Valine, μmol/L 138 ± 12 166 ± 21 127 ± 15 134 ± 12 133 ± 14 155 ± 12
  1. Values are means ± SEM where the sample size was 10, 10, 6, 7, 9, and 9 for the six groups, respectively. *Mature rats were administered either "low" dose alcohol (75 mmol/kg) or "high" dose alcohol (90 mmol/kg); young rats were injected with 75 mmol/kg. Blood was obtained from overnight fasted rats immediately prior to determination of muscle protein synthesis (e.g., time 0). Glucose and insulin concentrations were determined at time 0 and 10 min after injection of phenylalanine, and values averaged because there was no statistical difference between time points (data not shown). Values with different letters for a particular hormone are significantly different from each other, P < 0.05. Values with the same letter are not significantly different. HOMA = homeostasis model assessment for insulin resistance which is calculated as reported by Matthews et al [25].