RT-PCR of IL-5. For qualitative assessment, the PCR products were analyzed on 5% acrylamide gels stained with ethidium bromide. For quantitative analysis of IL-5, we used the Light Cycler quantitative PCR system. Based on the standard values of the control samples, the relative value for each test sample was determined with the Light Cycler software. In line 1 controls. In lines 2 & 3 treated allergic mice. In lines 4 & 5 non-treated allergic mice.