Effects of fatty acids on reactive oxygen species production by human corneal cells (H2DCF-DA test). The HCE-T cells were preincubated with fatty acids during 24 hours and then incubated with tBHP 50 μM or PBS during 1 hour. Mixtures DHA+mixed tocopherols and EPA+mixed tocopherols were also tested for their ability to decrease oxidative stress (hatched columns). tBHP induced an overproduction of ROS partially inhibited by the mixtures and oleic acid. *** p < 0.001, ** p < 0.01, * $p < 0.001.