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Figure 1 | Nutrition & Metabolism

Figure 1

From: n3 and n6 polyunsaturated fatty acids differentially modulate prostaglandin E secretion but not markers of lipogenesis in adipocytes

Figure 1

Effects of piroxicam and PGE 2 receptor agonists onadipose FAS activity in ApcMin/+ mice. Male C57BL/6J ApcMin/+ mice were maintained on the AIN-93G diet until approximately 80 days of age at which time they were randomly assigned to treatment groups. Treatments consisted of control, piroxicam (0.5 mg/mouse/day), EPR-A (16,16-dimethyl-PGE2 and 17-phenyl-trinor PGE2 -10 μg each), or piroxicam + EPR-A. Mice were sacrificed after 6 days of treatment and epididymal adipose tissue was harvested and snap frozen in liquid nitrogen. Tissue was homogenized in sucrose buffer and cytosolic extracts were analyzed for FAS activity using an activity assay as described in Materials and Methods. For treatments C and P + EPR-A n = 6; for P and EPR-A n = 5. Results represent the mean ± SEM. Values labeled with different letters are significantly different (p < 0.05). Values with the same letters do not differ significantly.

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