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Figure 4 | Nutrition & Metabolism

Figure 4

From: n3 and n6 polyunsaturated fatty acids differentially modulate prostaglandin E secretion but not markers of lipogenesis in adipocytes

Figure 4

Effects of COX-2 inhibition and EPA addition on PGE 2 levels (4A) and FAS activity (4B). 3T3 L-1 adipocytes were grown 6–7 days post-confluence, and starved for 24 h in serum-free media containing FA-free BSA. Fatty acids were incubated in media containing FA-free BSA for two hours at 37°C in a shaking water bath prior to treatment. Treatment consisted of CI (1 μM), OA, EPA, AA (150 μM each treatment), and AA + EPA (75 μM each FA). After 48 hours, 2 mL of media was removed, and cells were scraped using 350 μL of sucrose buffer, sonicated, and centrifuged for 1 hour. Media and cytosolic extracts were stored at -80°C. Media PGE2 levels and FAS activity were measured as described in the Materials and Methods. For all treatments n = 10. Results represent the mean ± SEM. Values labeled with different letters are significantly different (p < 0.05). Values with the same letters do not differ significantly.

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