Figure 6
Effect of exogenous PGE 2 and COX inhibition on adipocyte PGE 2 secretion (6A) and FAS activity (6B). 3T3 L-1 adipocytes were grown 6–7 days post-confluence, and starved for 24 h in serum free media containing FA free BSA. Treatment consisted of CI (1 μM), PGE2 (300 pM), and CI + PGE2. After 48 hours, 2 mL of media was removed and cells were scraped using 350 μL of sucrose buffer, sonicated, and centrifuged for 1 hour. Media and cytosolic extract were stored at -80° Media PGE2 levels and FAS activity were measured as described in the Materials and Methods. For all treatments n = 8. Results represent the mean ± SEM. Values labeled with different letters are significantly different (p < 0.05). Values with the same letters do not differ significantly.