Table 1 Recovery of [13C]LA or [13C]ALA after exposure of HepG2 cells to [13C]LA-/[13C]ALA-methylester at ratios of 0:1, 1:1, 4:1, 9:1 and 1:0 for 24 hours. 1not detected, LOD: 0.13 ng/μl.
LA/ALA 0:1 | LA/ALA 1:1 | LA/ALA 4:1 | LA/ALA 9:1 | LA/ALA 1:0 | |
|---|---|---|---|---|---|
13 C LA administered [μmol] | 0 | 50 | 80 | 90 | 100 |
cell culture medium [μmol] | n.d.1 | 7.1 ± 0.75 | 3.8 ± 0.35 | 4.5 ± 0.14 | 7.0 ± 0.37 |
cells [μmol] | n.d.1 | 37.6 ± 1.96 | 66.0 ± 2.51 | 75.3 ± 1.80 | 74.6 ± 2.97 |
percent administered [%] | - | 83 | 84 | 75 | 75 |
13 C ALA administered [μmol] | 100 | 50 | 20 | 10 | 0 |
cell culture medium [μmol] | 9.0 ± 1.2 | 4.7 ± 0.27 | 1.3 ± 0.11 | 2.1 ± 0.11 | n.d.1 |
cells [μmol] | 65.3 ± 3.90 | 32.6 ± 2.15 | 16.41 ± 0.31 | 6.90 ± 0.17 | n.d.1 |
Percent administered [%] | 65 | 65 | 82 | 69 | - |