Figure 1

Localization of HCV Core protein to lipid droplets. Huh-7 cells were transfected with a plasmid expressing the capsid (Core) protein from hepatitis C virus genotype 1 or genotype 3. Following transfection, cells were fixed, neutral lipids were stained by Red-Oil and core protein was localized by immunofluorescence using an anti-core primary and FITC-conjugated secondary and visualized on a confocal microscopy: 1A) Merged image demonstrating colocalization of HCV core protein (green) and neutral lipids (red) as intracellular lipid droplets 1B) FITC localized genotype 3 core protein and 1C) genotype 1.