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Figure 9 | Nutrition & Metabolism

Figure 9

From: Dietary fat and bile juice, but not obesity, are responsible for the increase in small intestinal permeability induced through the suppression of tight junction protein expression in LETO and OLETF rats

Figure 9

Immunoblot analysis of tight junction (TJ) proteins in the whole cell extracts of the Caco-2 cell monolayers incubated with or without rat bile juice (10 and 20%, A and B) and fat emulsion (0.5 and 1.0%, A and C) for 72 h. The whole cell extracts were prepared 72 h after the start of incubation and immunoblotted for occludin, claudin-1, claudin-3, junctional adhesion molecules-1 (JAM-1), and β-actin. Specific bands of occludin, claudin-1, claudin-3, and JAM-1 were quantitated by densitometric analysis. The density values were normalized to the value obtained for the control monolayers. Each value represents mean ± SEM, n = 6. Means without a common letter differ, P < 0.05. P values estimated by 1 way ANOVA were < 0.01 in each TJ protein expression in the cells treated with the rat bile juice (A) and fat emulsion (B).

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Nutrition & Metabolism

ISSN: 1743-7075

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