Table 1 Using 2H2O to study the effects of feeding and exercise on muscle protein synthesis
From: The application of 2H2O to measure skeletal muscle protein synthesis
Objective | Species | Assessment time | Outcome | Reference |
|---|---|---|---|---|
Determine if obesity impairs protein synthesis following nutrient ingestion | C57BL/6J mice | 5 hours | Diet-induced obesity impairs nutrient stimulated MPS | [26] |
Determine the effects of acute fasting & chronic food restriction on protein synthesis | Male Wistar rats (2-3 mo old) | 6 hours | Acute fasting (20 h) and chronic food restriction (7 d) impaired MPS | [27] |
Compare flooding dose vs. 2H2O to measure protein synthesis with acute resistance exercise | Male Sprague- Dawley rats (5-6 mo old) | 4 & 24 hours | Methodologies compare qualitatively, and more muscle protein is synthesized over 24 h vs. 4 h, however, synthesis rates were higher when measured over 4 hours | [20] |
Determine if chronic resistance exercise attenuates acute exercise stimulated protein synthesis | Male Sprague- Dawley rats (5-6 mo old) | 36 hours | Resistance exercise training alters the anabolic (MPS) response to acute exercise | [28] |
Determine the effects of high-intensity resistance exercise when energy & macronutrient content controlled | Male Humans (20-24 y old) | 24 hours | High-intensity resistance exercise stimulated myofibrillar, but not mixed MPS | [†] |