Figure 2

Myostatin precursor, dimer and FSTL-3 expression. (a) D140 female and male skeletal muscle tissue expression of myostatin precursor (50 kDa). Female ADAD chow (n = 3), ADUN chow (n = 4), UNAD chow (n = 7), ADAD high fat (n = 4), ADUN high fat (n = 4), UNAD high fat (n = 3); Male ADAD chow (n = 4), ADUN chow (n = 3), UNAD chow (n = 4), ADAD high fat (n = 2), ADUN high fat (n = 1), UNAD high fat (n = 5). (b) D140 female and male skeletal muscle tissue expression of myostatin dimer (28 kDa). Female ADAD chow (n = 6), ADUN chow (n = 7), UNAD chow (n = 7), ADAD high fat (n = 5), ADUN high fat (n = 6), UNAD high fat (n = 6); Male ADAD chow (n = 4), ADUN chow (n = 3), UNAD chow (n = 5), ADAD high fat (n = 4), ADUN high fat (n = 3), UNAD high fat (n = 5). (c) D140 female and male skeletal muscle tissue expression of FSTL-3 (27 kDa). Female ADAD chow (n = 3), ADUN chow (n = 6), UNAD chow (n = 7), ADAD high fat (n = 5), ADUN high fat (n = 6), UNAD high fat (n = 5); Male ADAD chow (n = 4), ADUN chow (n = 3), UNAD chow (n = 4), ADAD high fat (n = 3), ADUN high fat (n = 3), UNAD high fat (n = 5). Females are represented by black bars and males by the hatched bars. The graphs are divided on either side of the dashed line by postnatal diet (chow or high fat fed) of offspring. Gestational diet is on the x-axis and relative expression of the proteins on the y-axis. Data given as mean optical densities relative to positive control, sheep muscle ± SEM. Significance is shown by the stars where P < 0.05 *, P < 0.01 **, P < 0.001***.