Immunostaining of endothelial nitric oxide synthase (eNOS) in mesenteric artery homogenates from Chow (C), HSD (HS), and HFD (HF) fed rats. Densitized values of the immunoblot were normalized to β-actin protein concentration and are expressed as a ratio (mean ± SEM). Inset: representative blot of eNOS and β-actin in isolated rat mesenteric arteries from rats in all three treatment groups. n = 5-6 per group. Data are not significantly different.