Contribution of SVF cells isolated from epididymal fat depot to the expression of target mRNA in CLA mice. Epididymal adipose tissue was digested by collagenase and then separated into mature adipocytes and stromal vascular fraction. Expression levels of target genes of each fraction were measured by real time PCR and normalized by the internal housekeeping gene 18S rRNA. Expression data in the stromal vascular fraction (SVF), derived from equal amount of RNA (Table 4) were referred to the total RNA content in SVF. Data, means ± SEM of 6-8 mice, are represented as fold induction over control group. Mean values with unlike letters are significantly different (P < 0.01); ANOVA followed by LSD test.