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Table 4 Relative expression of target mRNAs in mature adipocytes and stromal vascular fraction in mice treated with CLA

From: Moderate doses of conjugated linoleic acid reduce fat gain, maintain insulin sensitivity without impairing inflammatory adipose tissue status in mice fed a high-fat diet

  Control CLA1 CLA2
Mature adipocytes    
   Adiponectin 1.00 ± 0.05a 0.72 ± 0.04b 0.39 ± 0.02c
   Leptin 1.00 ± 0.07a 0.57 ± 0.05b 0.16 ± 0.01c
   MCP1 1.00 ± 0.09a 1.28 ± 0.10b 0.71 ± 0.06c
Stromal Vascular Fraction    
   IL-6 1.00 ± 0.09a 0.96 ± 0.10a 0.61 ± 0.04b
   TNFα 1.00 ± 0.11a 1.47 ± 0.18ab 1.87 ± 0.30b
   iNOS 1.00 ± 0.08 0.81 ± 0.05 1.24 ± 0.20
   Emr1 1.00 ± 0.11a 1.46 ± 0.11a 2.64 ± 0.28b
   MCP1 1.00 ± 0.13 1.40 ± 0.18 1.23 ± 0.11
  1. Epididymal adipose tissue was digested by collagenase and then separated into mature adipocytes and stromal vascular fraction. Expression levels of target genes of each fraction were measured by real time PCR and normalized by the internal housekeeping gene 18S rRNA. The results, mean ± SEM of 6-8 mice/group, are expressed as fold induction over control group. Means in a row without a common letter differ, P < 0.05 (ANOVA followed by LSD test).