Skip to main content
Figure 2 | Nutrition & Metabolism

Figure 2

From: Identification of the Eph receptor pathway as a novel target for eicosapentaenoic acid (EPA) modification of gene expression in human colon adenocarcinoma cells (HT-29)

Figure 2

Effects of EPA and AA on EphB4 gene, Vav2 and EphA1 expression in HT29 cells as determined by real-time RT-PCR analysis. All data are expressed relative to β-Actin, allowing comparison to figure 3. Each data point represents the log of the mean and standard deviation of three replicate flasks of cells which were untreated, treated with EPA or AA over a 24 hr time course. (EPA closed squares, AA closed diamonds and untreated open circles). Significantly different values are represented by * (comparing EPA to untreated); ** (comparing EPA to AA). ‡ (comparing AA to both untreated and EPA). A) EphB4 expression is significantly affected by time (p < 0.02) and treatment p < 0.001). Post-hoc tests show significant treatment effect for AA (p < 0.001) and not EPA (p = 0.06). Individual time point analysis showed EPA significantly up-regulated EphB4 at 8 hr relative to AA (p = 0.05) and untreated (p = 0.09) and at 24 hr expression was still elevated but not significantly. B) Vav2 expression is significantly affected by time (p < 0.001) and treatment (p = 0.003) and the effect was associated with AA (p = 0.002). AA treatment reduced expression levels at 2 hr as compared to EPA (p = 0.005) and untreated (p = 0.05) but EPA treatment was non-significant compared to untreated cells (p = 0.1). At 24 hr Vav2 expression was higher in EPA treated than untreated cells but not significantly (p = 0.4). C) EphA1 expression is significantly affected by time (p = 0.01) and treatment (p < 0.001). At 4 hr there is a significant increase in gene expression (p = 0.006) and again at 24 hr (p = 0.01). This is associated with decreased expression of EphA1 in response to AA compared to EPA (p = 0.002) and untreated cells (p < 0.001) over the time course, however at individual time points no significant effect was detected.

Back to article page