Interventions in mice. 8 week-old male C57BL/6N mice were randomly assigned and subjected to one of the six interventions for 8 weeks: low-fat diet (LC, 10% fat), low-fat diet with 30% calorie restriction (LR), high-fat diet (HC, 60% fat), high-fat diet with 30% calorie restriction (HR), high-fat diet with voluntary running exercise (HE), and high-fat diet with a combination of 30% calorie restriction and exercise (HRE). (A) Average running distance of mice in the HE and HRE groups. (B) Average caloric intake of each mouse group. Data are shown as means ± SEM (n = 6/group). Statistical analyses were done with one-way ANOVA. *p < 0.05 HR vs. LC; N.S.: not significant.