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Table 2 Specific primers used for real-time quantitative PCR

From: Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue

Gene name1

Sequence

Ta

Product size (bp)

Genbank accession no.

18S rRNA

F: 5'-GCGGCTTTGGTGACTCTA-3'

R:5'-CTGCCTCCTTGGATGTG-3'

60°C

194

[238-431]

NR_002170.3

ACC

F: 5'-ATG TTT CGG CAG TCC CTG AT-3'

R: 5'-TGT GGA CCA GCT GAC CTT GA-3'

60°C

133

[4810-4942]

EF618729

FAS

F: 5'-AGC CTA ACT CCT CGC TGC AAT-3'

R: 5'-TCC TTG GAA CCG TCT GTG TTC-3'

58°C

196 bp

[504-699]

AY183428

SREBP-1

F: 5'-GCG ACG GTG CCT CTG GTA GT-3'

R: 5'-CGC AAG ACG GCG GAT TTA-3'

62°C

218 bp

[194-411]

AF102873

LPL

F: 5'-AAC TTG TGG CTG CCC TAT-3'

R: 5'-GAC CCT CTG GTG AAT GTG-3'

55°C

367 bp

[453-819]

X62984

HSL

F: 5'-GCT CCC ATC GTC AAG AAT C-3'

R: 5'-TAA AGC GAA TGC GGT CC-3'

55°C

262 bp

[2407-2668]

AJ000482

PPAR-γ

F: 5'-GAT TTC TCC AGC ATT TCC A-3'

R: 5'-GCT CTT CGT GAG GTT TGT T-3'

60°C

184 bp

[270-453]

DQ437884

CPT-1

F: 5'-ATG GTG GGC GAC TAA CT-3'

R: 5'-TGC CTG CTG TCT GTG AG-3'

62°C

321 bp

[681-1001]

AY181062

A-FABP

F: 5'-CAG GAA AGT CAA GAG CAC CA-3'

R: 5'-TCG GGA CAA TAC ATC CAA CA-3'

55°C

227 bp

[234-460]

AJ416020

  1. 1 All porcine gene sequences.