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Table 2 Specific primers used for real-time quantitative PCR

From: Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue

Gene name1 Sequence Ta Product size (bp) Genbank accession no.
18S rRNA F: 5'-GCGGCTTTGGTGACTCTA-3' R:5'-CTGCCTCCTTGGATGTG-3' 60°C 194 [238-431] NR_002170.3
ACC F: 5'-ATG TTT CGG CAG TCC CTG AT-3' R: 5'-TGT GGA CCA GCT GAC CTT GA-3' 60°C 133 [4810-4942] EF618729
FAS F: 5'-AGC CTA ACT CCT CGC TGC AAT-3' R: 5'-TCC TTG GAA CCG TCT GTG TTC-3' 58°C 196 bp [504-699] AY183428
SREBP-1 F: 5'-GCG ACG GTG CCT CTG GTA GT-3' R: 5'-CGC AAG ACG GCG GAT TTA-3' 62°C 218 bp [194-411] AF102873
LPL F: 5'-AAC TTG TGG CTG CCC TAT-3' R: 5'-GAC CCT CTG GTG AAT GTG-3' 55°C 367 bp [453-819] X62984
HSL F: 5'-GCT CCC ATC GTC AAG AAT C-3' R: 5'-TAA AGC GAA TGC GGT CC-3' 55°C 262 bp [2407-2668] AJ000482
PPAR-γ F: 5'-GAT TTC TCC AGC ATT TCC A-3' R: 5'-GCT CTT CGT GAG GTT TGT T-3' 60°C 184 bp [270-453] DQ437884
CPT-1 F: 5'-ATG GTG GGC GAC TAA CT-3' R: 5'-TGC CTG CTG TCT GTG AG-3' 62°C 321 bp [681-1001] AY181062
A-FABP F: 5'-CAG GAA AGT CAA GAG CAC CA-3' R: 5'-TCG GGA CAA TAC ATC CAA CA-3' 55°C 227 bp [234-460] AJ416020
  1. 1 All porcine gene sequences.