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Table 2 Specific primers used for real-time quantitative PCR

From: Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue

Gene name1 Sequence Ta Product size (bp) Genbank accession no.
18S rRNA F: 5'-GCGGCTTTGGTGACTCTA-3'
R:5'-CTGCCTCCTTGGATGTG-3'
60°C 194
[238-431]
NR_002170.3
ACC F: 5'-ATG TTT CGG CAG TCC CTG AT-3'
R: 5'-TGT GGA CCA GCT GAC CTT GA-3'
60°C 133
[4810-4942]
EF618729
FAS F: 5'-AGC CTA ACT CCT CGC TGC AAT-3'
R: 5'-TCC TTG GAA CCG TCT GTG TTC-3'
58°C 196 bp
[504-699]
AY183428
SREBP-1 F: 5'-GCG ACG GTG CCT CTG GTA GT-3'
R: 5'-CGC AAG ACG GCG GAT TTA-3'
62°C 218 bp
[194-411]
AF102873
LPL F: 5'-AAC TTG TGG CTG CCC TAT-3'
R: 5'-GAC CCT CTG GTG AAT GTG-3'
55°C 367 bp
[453-819]
X62984
HSL F: 5'-GCT CCC ATC GTC AAG AAT C-3'
R: 5'-TAA AGC GAA TGC GGT CC-3'
55°C 262 bp
[2407-2668]
AJ000482
PPAR-γ F: 5'-GAT TTC TCC AGC ATT TCC A-3'
R: 5'-GCT CTT CGT GAG GTT TGT T-3'
60°C 184 bp
[270-453]
DQ437884
CPT-1 F: 5'-ATG GTG GGC GAC TAA CT-3'
R: 5'-TGC CTG CTG TCT GTG AG-3'
62°C 321 bp
[681-1001]
AY181062
A-FABP F: 5'-CAG GAA AGT CAA GAG CAC CA-3'
R: 5'-TCG GGA CAA TAC ATC CAA CA-3'
55°C 227 bp
[234-460]
AJ416020
  1. 1 All porcine gene sequences.