Lotus leaf extract solution decreased triglyceride accumulation during preadipocyte/adipocyte differentiation. (A) Images after triglyceride staining (yellow) from cell populations incubated with and without 1% lotus leaf extract solution. Scale bar: 200 μm. (B) Triglyceride accumulation of maturing human adipocytes incubated with 0.5% or 1% (n = 12, each) lotus leaf extract solution relative to untreated control cells (n = 12) set as 100%. Results are depicted as mean ± SD. (C) Cell viability of maturing adipocytes cultivated in 'differentiation medium' with 0.5% or 1% lotus leaf extract solution (n = 12, each) compared to untreated control cells set as 100%. Results are depicted as mean ± SD. Significant differences are marked with an asterisk (* for p < 0.0001 against control).