Effect of lotus leaf extract solution on ADD1/SREBP-1c expression during adipogenesis. (A) ADD1/SREBP-1c gene expression in differentiating preadipocytes after incubation with 1% lotus leaf extract solution compared to control cells set as 100%. Expression of ADD1/SREBP-1c is normalized to GAPDH. Three independent experiments were prepared both for control and incubation with lotus leaf extract solution (n = 3). Results are depicted as mean ± SD. (B) Human preadipocyte populations were cultured in 'differentiation medium' without (a, c) or with (b, d) 1% lotus leaf extract solution for 9 days. Immunofluorescence staining of ADD1/SREBP-1c (red) and of DNA (Hoechst-33342 (blue)) was performed. a and b: Scale bar: 200 μm; c and d: Scale bar: 50 μm.