Figure 4

Rx significantly inhibited LPS-induced NF-κB activity (as indicated by SEAP activity) in mouse RAW-Blue macrophages. Different dosages of Rx (10 μg/ml or 20 μg/ml) or Rosiglitazone (10 μg/ml) together with 100 ng/ml LPS were incubated with RAW-Blue cells for 24 hours or 48 hours. DMSO was used as the vehicle control. The SEAP activities in the conditioned medium of mouse RAW-Blue cells were measured using QUANTI-Blue assay as described in Methods. Each bar represents the relative mean fold change ± SEM (n = 6). Data were statistically analyzed using one-way ANOVA with Dunnette's post hoc test. ** P < 0.01.