Rx treatment alleviated glucose intolerance and insulin resistance in db/db diabetic mice. Male db/db diabetic mice were treated with vehicle or Rx (2 g/kg/day) for 12 weeks by oral gavage. (a) Glucose tolerance test (GTT) conducted at week 12 after treatment. (b) Glucose tolerance test expressed as area under curve. (c) Insulin tolerance test (ITT) conducted at week 12 after treatment. (d) Insulin tolerance test expressed as area under curve. All mice were fasted for 16 hours or 6 hours before GTT and ITT were carried out, respectively. Data were statistically analyzed using one-way ANOVA with Dunnette's post hoc test. Data are presented as mean ± S.E.M. (n = 5-6). * P < 0.05; ** P < 0.01.