Escape latency (A), swimming distance (B) and swimming speed (C) over the training period. The animals were fed on the control diet (black diamond), or the control diet supplemented with 0.4 g/kg phytoestrogens (black square), 1.6 g/kg phytoestrogens (black triangle), or 0.15 g/kg 17βestradiol (white square) for 12 weeks. At the end of the experiment all animals were evaluated for their spatial memory performance with the Morris Water Maze task with a regime of 3 trials per day for 5 consecutive days. The data were presented as the means with the standard error bars (n = 7). Two-way ANOVA analysis showed significant differences in escape latency between different training days (F = 40.47, p < 0.001) and among treatment groups (F = 5.329, p < 0.01) with a significant interaction between the training time and the treatment (F = 2.558, p < 0.05). The analysis also showed significant differences in swimming distance between training days (F = 45.942, p < 0.001) and among treatment groups (F = 3.008, p < 0.05) with a significant interaction between the training time and the treatment (F = 3.063, p < 0.05). a, b: The mean values labelled with different letters differed significantly (p < 0.05). Significant differences from the mean values of the control on the same training day was indicated by * (p < 0.05).