Figure 4

Relative time spent and distance travelled in each of the quadrants in the probe test. (A) Relative time spent, (B) relative distance. The four quadrants of the swimming pool were the target quadrant (dark grey), the quadrant clockwise adjacent to the target quadrant (white), the quadrant anticlockwise adjacent to the target quadrant (grey spotted), and the quadrant opposite to the target quadrant (white spotted). The experimental animals were fed for 12 weeks on the control diet (Control), or diet supplemented with 0.4 g/kg phytoestrogens (Low-phyto), 1.6 g/kg phytoestrogens (High-phyto) or 0.15 g/kg 17β-estradiol (Estradiol). The probe test was performed 1 day after the last training trial. During the probe test the platform was removed from the pool while all other conditions remained the same as in the training trails. Values are means with their standard errors represented by vertical bars (n = 7). Significant differences from the mean values of the target quadrant in each group were indicated by *(p < 0.05); ** (p < 0.001).