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Table 2 Effect of dietary milk phospholipids in the form of phospholipid-rich dairy milk extract (PLRDME) on liver and fecal lipid parameters in mice given an intragastric gavage of radioactively labelled cholesterol and sitostanol after 3 or 5 weeks on high-fat diet

From: Hepatic accumulation of intestinal cholesterol is decreased and fecal cholesterol excretion is increased in mice fed a high-fat diet supplemented with milk phospholipids

 

3 weeks

5 weeks

Combined

 

HF

HFPL

difference

HF

HFPL

difference

HF

HFPL

difference

 

(n = 10)

(n = 8)

 

(n = 9)

(n = 10)

 

(n = 19)

(n = 18)

 

Liver

         

Total lipid (mg/organ)

118 ± 6

89 ± 10 *

-25%

212 ± 19

126 ± 7 ***

-41%

162 ± 14

110 ± 7 **

-32%

Cholesterol (μmol/organ)

33 ± 1

26 ± 3 *

-21%

64 ± 8

39 ± 4 **

-39%

48 ± 5

33 ± 3 *

-31%

Triglyceride (μmol/organ)

65 ± 5

42 ± 8 *

-35%

168 ± 14

90 ± 10 ***

-47%

114 ± 14

70 ± 9 *

-38%

[14C]cholesterol (dpm/organ)

6819 ± 836

3737 ± 948 *

-45%

13560 ± 1737

9021 ± 1043 *

-34%

10012 ± 1204

6673 ± 943 *

-33%

[3H]sitostanol (dpm/organ)

317 ± 31

244 ± 52

-23%

547 ± 41

370 ± 38 **

-32%

426 ± 37

318 ± 34 *

-25%

Feces

         

Total lipid (mg/mouse/day)

6.4 ± 0.6

10.6 ± 0.7 ***

+66%

9.2 ± 1.0

10.2 ± 1.1

+11%

7.8 ± 0.7

10.4 ± 0.7 *

+33%

Cholesterol (μmol/mouse/day)

7.3 ± 0.4

9.1 ± 0.5 *

+25%

6.7 ± 0.3

7.4 ± 0.4

+11%

7.0 ± 0.3

8.1 ± 0.4 *

+16%

Triglyceride (μmol/mouse/day)

0.9 ± 0.1

1.0 ± 0.2

+11%

2.6 ± 0.5

2.0 ± 0.4

-24%

1.8 ± 0.3

1.6 ± 0.3

-11%

[14C]cholesterol (dpm/mouse/day)

9195 ± 1187

13454 ± 996 *

+46%

8889 ± 795

10245 ± 864

+15%

9050 ± 711

11765 ± 740 *

+30%

[3H]sitostanol (dpm/mouse/day)

12376 ± 1275

17110 ± 712 **

+38%

16076 ± 570

18134 ± 777

+13%

14129 ± 828

17679 ± 535 **

+25%

  1. Results represent means ± SEM. Mice given the high-fat diet alone were designated HF. Mice given the high-fat diet supplemented with 2.5% by wt PLRDME were designated HFPL. Data are provided for animals fed for 3 or 5 weeks. Combined data are shown on the right. Percentage difference between groups is given in italics. Significant difference between HF and HFPL by Student's t-test: * P < 0.05, ** P < 0.01, *** P < 0.001.