Figure 2

Effects of bilberry extracts on the expression of adipogenesis-related transcription factors. 3T3-L1 preadipocytes were incubated with DMSO or 100 μ g/mL of bilberry extracts and induced to differentiate. The expression levels of (A) master regulators of adipocyte differentiation; Ppar γ , C/ebp α , (B) upstream genes; C/ebp β , C/ebp δ , Klf5 and Srebp1c at various time points after induction were measured by real-time RT-PCR (n = 3). The results are expressed as the fold increase relative to the controls after normalizing to the β -actin expression levels. The results are shown as the mean ± S.D. (* p < 0.05, ** p < 0.001, Welch's t-test).