Figure 2
Kinetics of insulin secretion. Panel a shows the kinetics of insulin secretion from in situ perfusion of pancreas in response to glucose (Glc) in refed and control animals (n = 6) on day 7 of refeeding, first in response to 2.8 mM glucose (baseline), followed by two successive periods lasting 15 min each in response to 16.7 mmol/l glucose before switching back to 2.8 mmol/l glucose. Panel b shows the area under the curve (AUC) for each time-period, calculated after subtraction of basal release. All values are mean ± SE. Symbols for statistical significance of differences are as follows: panel a: * p < 0.05 (at least) between refed and control for corresponding time points; Panel b: * p < 0.05: between-group comparison (refed vs control) for AUC within 1st or 2nd period; § p < 0.05; §§ p < 0.01: within-group comparison between 16.7 mM Glc or 8 mM relative to basal 2.8 mM Glc. Panel c indicates the kinetics of insulin secretion from ex vivo perifusion of islets isolated from pancreases of refed and control groups (n = 5) on day 7 of refeeding, first in response to 2.8 mmol/l glucose (baseline), followed by two successive periods of 17 min with 8 and 16.7 mmol/l glucose, respectively, before switching back to 2.8 mmol/l glucose; panel d shows the AUC for each time-period, calculated after subtraction of basal release. Panel d shows the area under the curve (AUC) for each time-period, calculated after subtraction of basal release All values are mean ± SE. Symbols for statistical significance of differences in panel b are as follows: § p < 0.05: within-group comparison between 16.7 mM Glc or 8 mM Glc relative to basal 2.8 mM Glc