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Table 1 Protocol parameters differing from the standard SpotDetector® algorithm

From: Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets

Object Identification

  

Channel 1

Method:

FixedThreshold

 

Value:

125

Channel 2

Method:

TriangThreshold

 

Value:

0.499

Object Selection Parameter

Min

Max

Channel 1

ObjectArea:

100

6000

Channel 2

SpotAreaCh2:

3

10000

 

SpotAvgIntenCh2:

30

4095

 

SpotTotalIntenCh2:

1691

4411431044

Assay Parameters

  
 

BackgroundCorrection:

140

 

ObjectSegmentationCh1:

20

 

SmoothFactorCh1:

5

 

SpotDetectRadiusCh2:

15

 

TargetCircModifierCh2:

512

 

PixelSize:

0.256 microns

  1. The alteration of assay parameters enables adjustment of the algorithm to different biological cell models. Displayed values are pixel number.
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Nutrition & Metabolism

ISSN: 1743-7075

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