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Table 1 Protocol parameters differing from the standard SpotDetector® algorithm

From: Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets

Object Identification   
Channel 1 Method: FixedThreshold
  Value: 125
Channel 2 Method: TriangThreshold
  Value: 0.499
Object Selection Parameter Min Max
Channel 1 ObjectArea: 100 6000
Channel 2 SpotAreaCh2: 3 10000
  SpotAvgIntenCh2: 30 4095
  SpotTotalIntenCh2: 1691 4411431044
Assay Parameters   
  BackgroundCorrection: 140
  ObjectSegmentationCh1: 20
  SmoothFactorCh1: 5
  SpotDetectRadiusCh2: 15
  TargetCircModifierCh2: 512
  PixelSize: 0.256 microns
  1. The alteration of assay parameters enables adjustment of the algorithm to different biological cell models. Displayed values are pixel number.