|
Object Identification
| | |
|---|
|
Channel 1
|
Method:
|
FixedThreshold
|
| |
Value:
|
125
|
|
Channel 2
|
Method:
|
TriangThreshold
|
| |
Value:
|
0.499
|
|
Object Selection Parameter
|
Min
|
Max
|
|
Channel 1
|
ObjectArea:
|
100
|
6000
|
|
Channel 2
|
SpotAreaCh2:
|
3
|
10000
|
| |
SpotAvgIntenCh2:
|
30
|
4095
|
| |
SpotTotalIntenCh2:
|
1691
|
4411431044
|
|
Assay Parameters
| | |
| |
BackgroundCorrection:
|
140
|
| |
ObjectSegmentationCh1:
|
20
|
| |
SmoothFactorCh1:
|
5
|
| |
SpotDetectRadiusCh2:
|
15
|
| |
TargetCircModifierCh2:
|
512
|
| |
PixelSize:
|
0.256 microns
|
- The alteration of assay parameters enables adjustment of the algorithm to different biological cell models. Displayed values are pixel number.
