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Table 2 Sequences of primers and probes for different adipocyte specific genes used in real-time RT-PCR

From: Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets

Gene name Forward/Reverse Primer Probe
Adiponectin (Acrp30) 5'-GGCACTCCTGGAGAGAAGGG
5'-ATTCCAACATCTCCTGTCTCACC
5'-GAAAGGAGATGCAGGTCTTCTTGGTCCTA
Fatty acid binding protein 4, adipocyte type (aP2) 5'-GCGTGGAATTCGATGAAATCA
5'-CCCGCCATCTAGGGTTATGA
5'-CGCAGACGACAGGAAGGTGAAGAGC
Acetyl-CoA carboxylase (ACC1) 5'-TCACTCGCTTTGGAGGCAA
5'-CGCAGCGATGCCATTGT
5'-AGGGTCATAGAGAAGGTGCTCATCGCC
Lipoprotein lipase (LPL) 5'-GTGGCCGAGAGCGAGAAC
5'-AAGAAGGAGTAGGTTTTATTTGTGGAA
5'-TTCCCTTCACCCTGCCCGAGG
Hormone sensitive lipase (HSL) 5'-AAGACCACATCGCCCACAG
5'-CAGACACACTCCTGCGCATAG
5'-AGAGTCTGTGCGCCCCACGGA
Fatty acid synthase (FAS) 5'-TCATAAAGCAGTTTCTTGATGTGGA
5'-CAGGCTCTTCAGTGGCAGC
5'-CACAGCAAGGTGCTGGAGGCCC
Carnitine palmitoyl transferase β (CPT-1 β) 5'-CCAATCATCTGGGTGCTGG
5'-TAAGAGACCCCGTAGCCATCAT
5'-TGGCTTTGGTCCCGTGGCG
Glucose transporter 4 (Glut4) 5'-TCGGCTCTGACGATGGG
5'-CCAAGCCAGCTGAGAATACAG
5'-AACCCCCTCGGCAGCGAGTGACTG
Peroxisome proliferator activated receptor gamma 2a (PPARγ 2a) 5'-CTATGAGCACTTCACAAGAAATTACCAT
5'-TCCATCACGGAGAGGTCCAC
5'- TCTGGCCCACCAACTTCGGAATCAG
Peroxisome proliferator activated receptor alpha (PPARα) 5'-GCCTCAGGGTACCACTACGG
5'-GCCGAATAGTTCGCCGAA
5'-CACGCATGTGAAGGCTGTAAGGGCTT
CCAAT/enhancer binding protein alpha (C/EBPα) 5'-CGGTGCGGGCAAAGC
5'-TGCGTTCCCGCCGTAC
5'-AGAAGTCGGTGGACAAGAACAGCAAC
18S rRNA 5'-CGGCTACCACATCCAAGG
5'-CGGGTCGGGAGTGGGT
5'-TTGCGCGCCTGCTGCCT