Table 2 Sequences of primers and probes for different adipocyte specific genes used in real-time RT-PCR
Gene name | Forward/Reverse Primer | Probe |
|---|---|---|
Adiponectin (Acrp30) | 5'-GGCACTCCTGGAGAGAAGGG 5'-ATTCCAACATCTCCTGTCTCACC | 5'-GAAAGGAGATGCAGGTCTTCTTGGTCCTA |
Fatty acid binding protein 4, adipocyte type (aP2) | 5'-GCGTGGAATTCGATGAAATCA 5'-CCCGCCATCTAGGGTTATGA | 5'-CGCAGACGACAGGAAGGTGAAGAGC |
Acetyl-CoA carboxylase (ACC1) | 5'-TCACTCGCTTTGGAGGCAA 5'-CGCAGCGATGCCATTGT | 5'-AGGGTCATAGAGAAGGTGCTCATCGCC |
Lipoprotein lipase (LPL) | 5'-GTGGCCGAGAGCGAGAAC 5'-AAGAAGGAGTAGGTTTTATTTGTGGAA | 5'-TTCCCTTCACCCTGCCCGAGG |
Hormone sensitive lipase (HSL) | 5'-AAGACCACATCGCCCACAG 5'-CAGACACACTCCTGCGCATAG | 5'-AGAGTCTGTGCGCCCCACGGA |
Fatty acid synthase (FAS) | 5'-TCATAAAGCAGTTTCTTGATGTGGA 5'-CAGGCTCTTCAGTGGCAGC | 5'-CACAGCAAGGTGCTGGAGGCCC |
Carnitine palmitoyl transferase β (CPT-1 β) | 5'-CCAATCATCTGGGTGCTGG 5'-TAAGAGACCCCGTAGCCATCAT | 5'-TGGCTTTGGTCCCGTGGCG |
Glucose transporter 4 (Glut4) | 5'-TCGGCTCTGACGATGGG 5'-CCAAGCCAGCTGAGAATACAG | 5'-AACCCCCTCGGCAGCGAGTGACTG |
Peroxisome proliferator activated receptor gamma 2a (PPARγ 2a) | 5'-CTATGAGCACTTCACAAGAAATTACCAT 5'-TCCATCACGGAGAGGTCCAC | 5'- TCTGGCCCACCAACTTCGGAATCAG |
Peroxisome proliferator activated receptor alpha (PPARα) | 5'-GCCTCAGGGTACCACTACGG 5'-GCCGAATAGTTCGCCGAA | 5'-CACGCATGTGAAGGCTGTAAGGGCTT |
CCAAT/enhancer binding protein alpha (C/EBPα) | 5'-CGGTGCGGGCAAAGC 5'-TGCGTTCCCGCCGTAC | 5'-AGAAGTCGGTGGACAAGAACAGCAAC |
18S rRNA | 5'-CGGCTACCACATCCAAGG 5'-CGGGTCGGGAGTGGGT | 5'-TTGCGCGCCTGCTGCCT |