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Figure 2 | Nutrition & Metabolism

Figure 2

From: The Regulation of Leptin, Leptin Receptor and Pro-opiomelanocortin Expression by N-3 PUFAs in Diet-Induced Obese Mice Is Not Related to the Methylation of Their Promoters

Figure 2

The effects of n-3 PUFAs on the mRNA expression of leptin, leptin-R, POMC and NPY in DIO mice. Male C57BL/6J mice, 3-4 weeks old, were fed one of four DIO diets with different fat types or a control diet for 3 months. Real-time quantitative RT-PCR was used to measure the mRNA levels of leptin in epididymal fat and those of leptin-R, POMC and NPY in the hypothalamus. The data were normalized to GAPDH mRNA levels using the 2ΔΔCT method. Values are means ± SD, n = 15 in each group. * compared to the control diets, P < 0.05; compared to the n-3 deficient DIO diet, P < 0.05.

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