The effects of n-3 PUFAs on the mRNA expression of leptin, leptin-R, POMC and NPY in DIO mice. Male C57BL/6J mice, 3-4 weeks old, were fed one of four DIO diets with different fat types or a control diet for 3 months. Real-time quantitative RT-PCR was used to measure the mRNA levels of leptin in epididymal fat and those of leptin-R, POMC and NPY in the hypothalamus. The data were normalized to GAPDH mRNA levels using the 2ΔΔCT method. Values are means ± SD, n = 15 in each group. * compared to the control diets, P < 0.05; ‡ compared to the n-3 deficient DIO diet, P < 0.05.