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Table 1 Composition of the animal diets

From: The Regulation of Leptin, Leptin Receptor and Pro-opiomelanocortin Expression by N-3 PUFAs in Diet-Induced Obese Mice Is Not Related to the Methylation of Their Promoters

  Control DIO
  n-3 deficient Soy oil n-3 deficient Soy oil Soy/fish oil Fish oil
Components (g/kg)
   Lard 20 20 318 318 318 318
   Sunflower oil 25 - 35 - - -
   Soy oil - 25 - 35 17.5 -
   Fish oil - - - - 17.5 35
Fatty acid composistion (% of fat)
   Σ Saturated 27.22 28.33 41.83 42.03 43.02 44.01
   Σ Momounsaturated 30.67 33.33 41.62 42.82 43.24 42.81
   18:2n-6 41.14 32.39 15.51 13.36 10.65 8.59
   20:4n-6 0.97 1.05 0.94 0.7 0.58 0.62
   22:4n-6 - - - 0.3 0.6 0.9
   Σ n-6 PUFAs 42.11 34.44 16.55 14.36 11.83 10.11
   18:3n-3 0.09 3.89 0.12 0.79 0.17 0.11
   20:5n-3 - - - - 1.02 1.85
   22:6n-3 - - - - 0.72 1.11
   Σ n-3 PUFAs 0.09 3.89 0.12 0.79 1.91 3.07
Total energy (kcal%)
   Fat 10 10 60 60 60 60
   Protein 20 20 20 20 20 20
   Carbohydrate 70 70 20 20 20 20