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Table 2 Bisulfite sequencing primers used in this study

From: The Regulation of Leptin, Leptin Receptor and Pro-opiomelanocortin Expression by N-3 PUFAs in Diet-Induced Obese Mice Is Not Related to the Methylation of Their Promoters

Gene name Forward (F) and Reverse (R) Primers Product size (bp)
Leptin   310
  Outer F: 5'-GAGTAGTTAGGTTAGGTATGTAAAGAG-3'  
  Inner F: 5'-AGTTTTTTGTAGTTTTTTGTTTTTTG-3'  
     R: 5'-TAATAACTACCCCAATACCACTTAC-3'  
Leptin-R   294
     F: 5'-TGTAAAGGTTAGAGAGGATAGAATTAG-3'  
  Outer R: 5'-CTTATATCTTTCAAACCAACCCACCCT-3'  
  Inner R: 5'-CCAATCTACTACCTAATCCCCAAAC-3'  
POMC   258
  Outer F: 5'-TTGTAAGATTTTAGAATTAGGTTTG-3'  
  Inner F: 5'-AGTTTTTGTTTAGTTTTAAGTGGAG-3'  
     R: 5'-CTCTCTTCTTTTATACTTACAAAATTAAA-3'