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Table 4 Quantitative methylation analysis of the promoter region of the leptin gene.

From: The Regulation of Leptin, Leptin Receptor and Pro-opiomelanocortin Expression by N-3 PUFAs in Diet-Induced Obese Mice Is Not Related to the Methylation of Their Promoters

Diets CpG site (position) Total
  a b c d e f g h i j k l m n o p  
DIO                  
n-3 deficient 66 80 63 71 80 68 78 83 67 80 77 81 82 78 89 93 77
Soy oil 65 78 63 69 79 68 80 83 66 78 79 81 82 81 90 95 77
Soy/Fish oil 62 78 62 68 77 66 78 82 65 76 76 79 81 79 89 96 76
Fish oil 63 75 61 66 75 66 77 82 64 76 76 79 80 78 88 93 75
Control                  
n-3 deficient 58 69 57 65 74 61 75 80 67 71 70 74 78 74 88 93 72
Soy oil 60 73 56 64 72 63 74 78 63 73 73 77 80 78 87 94 73
  1. 3-4 weeks old C57BL/6J male mice were fed four DIO diets with different fat types and the control diet for 3 months. Genomic DNA isolated from epididymal fat was analyzed for methylation status of 16 CpG sites at the indicated positions of the leptin gene promoter spanning nucleotides from -324 to -29 relative to transcription start site. Data for each CpG site represent the mean percentage level (0% = nonmethylated, 100% = methylated) determined from 15 mice in each group.