The Regulation of Leptin, Leptin Receptor and Pro-opiomelanocortin Expression by N-3 PUFAs in Diet-Induced Obese Mice Is Not Related to the Methylation of Their Promoters

Table 4 Quantitative methylation analysis of the promoter region of the leptin gene.

3-4 weeks old C57BL/6J male mice were fed four DIO diets with different fat types and the control diet for 3 months. Genomic DNA isolated from epididymal fat was analyzed for methylation status of 16 CpG sites at the indicated positions of the leptin gene promoter spanning nucleotides from -324 to -29 relative to transcription start site. Data for each CpG site represent the mean percentage level (0% = nonmethylated, 100% = methylated) determined from 15 mice in each group.

ISSN: 1743-7075