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Table 5 Quantitative methylation analysis of the promoter region of the POMC gene.

From: The Regulation of Leptin, Leptin Receptor and Pro-opiomelanocortin Expression by N-3 PUFAs in Diet-Induced Obese Mice Is Not Related to the Methylation of Their Promoters

Diets CpG site (position) Total
  a b c d e f g h i j k l m n  
DIO                
n-3 deficient 81 80 61 71 75 81 80 47 50 75 63 63 71 73 69
Soy oil 80 80 61 73 78 83 81 48 48 77 67 65 75 76 71
Soy/Fish oil 81 81 62 73 78 82 81 48 49 75 64 62 72 73 70
Fish oil 78 77 57 71 79 83 82 45 52 76 56 65 73 75 69
Control                
n-3 deficient 80 80 61 72 78 81 80 47 50 76 65 63 73 73 70
Soy oil 78 78 58 70 78 81 81 46 49 76 64 65 74 75 70
  1. 3-4 week old C57BL/6J male mice were fed four DIO diets with different fat types and the control diet for 3 months. Genomic DNA isolated from epididymal fat tissue was analyzed for methylation status of 14 CpG sites at the indicated positions of the POMC gene promoter spanning nucleotides -660 to -53 relative to transcription start site. Data for each CpG site represent the mean percentage level (0% = nonmethylated, 100% = methylated) determined from 15 mice in each group.