Evaluation of caveolin-1 expression. The RPE cells were incubated with culture medium (left chart) or YS-2636 oil (right chart) for 15 minutes. After the cells were rinsed with phosphate buffer, the cells were incubated with culture medium for 24 hours and fixed in PFA. The expression of caveolin-1 was quantified using flow cytometry. Figures are representative of three independent experiments.