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Figure 4 | Nutrition & Metabolism

Figure 4

From: Dietary cholesterol, female gender and n-3 fatty acid deficiency are more important factors in the development of non-alcoholic fatty liver disease than the saturation index of the fat

Figure 4

The concentration of triglycerides in liver. Panel A shows the triglyceride concentration measured enzymatically. Panel C indicates the percentage of fat present as >2 μm (macrovesicular) globules determined morphometrically in Oil-red O-stained sections. The correlation of the biochemical assay (Panel A) and the histochemical assay (Panel C) is shown in panel B (P < 0.001). Assuming ~150 mg protein per gram liver wet weight, 1 vol% ORO represents ~4 μmol TG/gram liver. Panel D: The concentration of mead acid in the liver TG fraction correlates positively with the hepatic triglyceride concentration. The grey triangles indicate hoSO- and the grey circles OO-fed mice, with the APOE2ki mice emphasized with a black contour; all other small black dots indicate the other diet groups. Note the very high mead acid concentrations in hoSO-fed APOE2ki compared to WT mice. Also note that livers with a low fat content have no detectable mead acid levels. Panel E: Fasn, Srebf1, and Scd1 mRNA expression in the liver of hoSO and olive oil-fed APOE2ki mice. Panel F compares the composition of liver fat as determined by gas chromatography and the dietary fatty acid composition (D: fatty-acid composition in diet; L: fatty-acid composition in liver triglycerides). Values are indicated as means ± SEM. Different letters indicate a significant difference (P < 0.05) within each group. GT: genotype; n.s.: non significant.

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Nutrition & Metabolism

ISSN: 1743-7075

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