Figure 4

Reversibility of CZ action. α-glucosidase (100 U) was incubated with CZ (23.5 mg) in 0.5 ml of sodium phosphate buffer (50 mM; pH 6.7) for 2 h at 37°C and dialyzed against sodium phosphate buffer (5 mM; pH 6.7) at 4°C for 24 h. Reversibility of CZ was determined by comparing the residual enzyme activity after dialysis with that of non-dialyzed one. α-glucosidase alone (EC, ED) and the complex of α-glucosidase and CZ (EIC, EID) were dialyzed against 5 mM sodium phosphate buffer (pH 6.7) at 4°C (ED, EID) or were kept at 4°C (EC, EIC) for 24 h.