CZ (mg/ml) | Vmax (mM/min) | Km (mM) |
---|
0 | 0.94 | 0.85 |
0.5 | 0.94 | 1.22 |
1 | 0.94 | 1.59 |
- Table 1. α-glucosidase with different concentrations of PNPG (0.5-4 mM) was incubated in the absence and presence of CZ at two different concentrations (0.5 and 1 mg/ml) at 37°C for 30 min. Km and Vmax were calculated from Lineweaver-Burk plot.