Figure 1

Representative example of targeted profiling of diacylglycerols (DAGs) in lipid extracts using multi-dimensional mass spectrometry with LC-ESI-IT-ToF. A: Representative extracted ion chromatogram of a pre-purified liver lipid extract containing various DAG species (22:6-22:6, 18:2-22:6, 18:1-22:6, 16:0-22:6), including the biosynthetic 2-AG precursors that were measured here (18:1-20:4, 16:0-20:4, 18:0-20:4). B: Example of a positive-ion electrospray mass spectrum of a major component of the extract, the 18:0-20:4 DAG precursor ion (m/z 667.5273), as a sodium adduct, and the corresponding product ions for the CID of the fragment with m/z 667.5273 in the MS² spectra (m/z 327.2295 and 383.2562), which in turn correspond to the sodiated 20:4 acid and C₂₃H₃₆O₃ fragments, respectively.