| |
N
|
HF
|
HFKO1.25
|
HFKO2.5
|
HFKO5
|
|---|
|
Initial weight (g)
|
18.1 ± 0.3
|
18.5 ± 0.8
|
18.0 ± 0.3
|
18.2 ± 0.6
|
18.3 ± 0.8
|
|
Final weight (g)
|
24.6 ± 0.3
|
31.7 ± 0.9###
|
31.0 ± 0.9
|
31.8 ± 0.7
|
32.4 ± 1.0
|
|
Weight gain
|
6.5 ± 0.4
|
13.3 ± 1.1###
|
13.0 ± 0.9
|
13.6 ± 0.7
|
14.1 ± 0.6
|
|
TG (mmol/L)
|
0.4 ± 0.03
|
0.6 ± 0.06##
|
0.8 ± 0.08
|
0.8 ± 0.09
|
0.8 ± 0.06
|
|
Cholesterol (mmol/L)
|
2.6 ± 0.1
|
4.3 ± 0.3###
|
3.4 ± 0.1a
|
3.0 ± 0.1b
|
3.0 ± 0.2b
|
|
PL (mmol/L)
|
1.3 ± 0.1
|
1.7 ± 0.1###
|
1.5 ± 0.1a
|
1.3 ± 0.1c
|
1.3 ± 0.1c
|
|
Glucose (mmol/L)
|
11.5 ± 0.6
|
13.1 ± 1.6
|
8.3 ± 0.6b
|
8.7 ± 0.8a
|
7.5 ± 0.8b
|
|
Insulin (mmol/L)
|
1.1 ± 0.3
|
0.7 ± 0.1
|
0.6 ± 0.1
|
0.8 ± 0.1
|
0.7 ± 0.1
|
|
Adiponectin (μg/ml)
|
4.7 ± 0.2
|
5.0 ± 0.1
|
6.5 ± 0.3
|
6.5 ± 0.4
|
7.5 ± 0.6a
|
|
Fatty acid synthase
|
109 ± 15
|
44 ± 9
##
|
27 ± 4
|
13 ± 1
c
|
17 ± 2
c
|
|
Acetyl-CoA carboxylase
|
104 ± 10
|
67 ± 10
##
|
50 ± 5
|
28 ± 3
c
|
32 ± 4
b
|
|
Stearoyl-CoA desaturase
|
106 ± 11
|
232 ± 43
##
|
154 ± 18
|
55 ± 8
c
|
78 ± 16
c
|
|
CD36
|
101 ± 5
|
232 ± 28
###
|
256 ± 11
|
131 ± 13
|
219 ± 38
|
|
Monoacylglycerol lipase
|
102 ± 7
|
146 ± 17
#
|
114 ± 9
|
50 ± 4
c
|
68 ± 7
b
|
- Data are taken from (30) and are means ± SEM of separate determinations in N = 6-10 mice. ## P < 0.01 and ### P < 0.001 N vs HF group; a P < 0.05, b P < 0.01 and c P < 0.001 HF vs HFKO groups. Expression values represent mRNA levels expressed as a ratio relative to cyclophilin, and then expressed as percent of those in N mice. Results represent means ± SEMs (n = 6-10 mice per group). Mice were fed diets for 8 weeks. Significant difference between N and HF by Student's t-test: #P < 0.05, ##P < 0.01, and ###P < 0.001. Significant difference between HF- and HFKO-supplemented animals by ANOVA followed by Tukey's test for significance. a, P < 0.05; b, P < 0.01, and c, P < 0.001. Data and corresponding statistical analyses are from [30] and are reported here to show how changes in the endocannabinoidome induced by dietary ω-3 fatty acids is associated with metabolic changes.
