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Table 3 Metabolic parameters and expression of some hepatic metabolic genes (italics) in mice fed the experimental diets

From: Effect of dietary krill oil supplementation on the endocannabinoidome of metabolically relevant tissues from high-fat-fed mice

  N HF HFKO1.25 HFKO2.5 HFKO5
Initial weight (g) 18.1 ± 0.3 18.5 ± 0.8 18.0 ± 0.3 18.2 ± 0.6 18.3 ± 0.8
Final weight (g) 24.6 ± 0.3 31.7 ± 0.9### 31.0 ± 0.9 31.8 ± 0.7 32.4 ± 1.0
Weight gain 6.5 ± 0.4 13.3 ± 1.1### 13.0 ± 0.9 13.6 ± 0.7 14.1 ± 0.6
TG (mmol/L) 0.4 ± 0.03 0.6 ± 0.06## 0.8 ± 0.08 0.8 ± 0.09 0.8 ± 0.06
Cholesterol (mmol/L) 2.6 ± 0.1 4.3 ± 0.3### 3.4 ± 0.1a 3.0 ± 0.1b 3.0 ± 0.2b
PL (mmol/L) 1.3 ± 0.1 1.7 ± 0.1### 1.5 ± 0.1a 1.3 ± 0.1c 1.3 ± 0.1c
Glucose (mmol/L) 11.5 ± 0.6 13.1 ± 1.6 8.3 ± 0.6b 8.7 ± 0.8a 7.5 ± 0.8b
Insulin (mmol/L) 1.1 ± 0.3 0.7 ± 0.1 0.6 ± 0.1 0.8 ± 0.1 0.7 ± 0.1
Adiponectin (μg/ml) 4.7 ± 0.2 5.0 ± 0.1 6.5 ± 0.3 6.5 ± 0.4 7.5 ± 0.6a
Fatty acid synthase 109 ± 15 44 ± 9 ## 27 ± 4 13 ± 1 c 17 ± 2 c
Acetyl-CoA carboxylase 104 ± 10 67 ± 10 ## 50 ± 5 28 ± 3 c 32 ± 4 b
Stearoyl-CoA desaturase 106 ± 11 232 ± 43 ## 154 ± 18 55 ± 8 c 78 ± 16 c
CD36 101 ± 5 232 ± 28 ### 256 ± 11 131 ± 13 219 ± 38
Monoacylglycerol lipase 102 ± 7 146 ± 17 # 114 ± 9 50 ± 4 c 68 ± 7 b
  1. Data are taken from (30) and are means ± SEM of separate determinations in N = 6-10 mice. ## P < 0.01 and ### P < 0.001 N vs HF group; a P < 0.05, b P < 0.01 and c P < 0.001 HF vs HFKO groups. Expression values represent mRNA levels expressed as a ratio relative to cyclophilin, and then expressed as percent of those in N mice. Results represent means ± SEMs (n = 6-10 mice per group). Mice were fed diets for 8 weeks. Significant difference between N and HF by Student's t-test: #P < 0.05, ##P < 0.01, and ###P < 0.001. Significant difference between HF- and HFKO-supplemented animals by ANOVA followed by Tukey's test for significance. a, P < 0.05; b, P < 0.01, and c, P < 0.001. Data and corresponding statistical analyses are from [30] and are reported here to show how changes in the endocannabinoidome induced by dietary ω-3 fatty acids is associated with metabolic changes.