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Table 4 Effect of diet on fatty acid composition of liver of different mice lines

From: Metabolic responses to high-fat diets rich in n-3 or n-6 long-chain polyunsaturated fatty acids in mice selected for either high body weight or leanness explain different health outcomes

 

DU6

DUhTP

 

Standard chow

n-3 HFD

n-6 HFD

Standard chow

n-3 HFD

n-6 HFD

 

LSM ± SE

LSM ± SE

LSM ± SE

LSM ± SE

LSM ± SE

LSM ± SE

SFA+

2420 ± 222

2794 ± 251

2981 ± 210

1889 ± 222

2520 ± 210

2224 ± 222

C14:0

23·2 ± 8a

73·1 ± 9b

41·2 ± 7a, b

20·0 ± 8a

77·7 ± 7b

23·4 ± 8a

C16:0

1888 ± 186

2057 ± 211

2292 ± 177A

1335 ± 186

1721 ± 177

1351 ± 186B

C18:0

435·3 ± 33

560·1 ± 38

547·5 ± 32A

436·3 ± 33a

589·5 ± 32b

733·6 ± 33c, B

MUFA#

2074 ± 225A

1567 ± 256

2544 ± 214A

1006 ± 225B

1473 ± 214

1547 ± 226B

C16:1cis-9

218·4 ± 26

247·8 ± 29

143·5 ± 24

110·1 ± 26a

244·6 ± 24b

89·9 ± 25a

C18:1cis- 9

1636 ± 184a, b, A

1178 ± 209a

2225 ± 175b, A

793 ± 185B

1044 ± 175

1355 ± 185B

∑n-6 FAd

2706 ± 438a

754·6 ± 497b

6135 ± 416c

2283 ± 438a

759·7 ± 416b

5876 ± 438a

C18:2n-6

1941 ± 269a

474·8 ± 47b

5003 ± 575c

1686 ± 349a

478 ± 39b

4605 ± 587c

C20:4n-6

557·7 ± 38a

226·8 ± 43b

727·2 ± 37cA

479·3 ± 39a

236·9 ± 37b

902·9 ± 39cB

∑n-3 FAe

505·0 ± 144a

2385 ± 163b

237·9 ± 136a

556·6 ± 144a

2197 ± 136b

110·1 ± 144a

C18:3n- 3

70·4 ± 11a

93·3 ± 13a

13·3 ± 11b

63·2 ± 11a

85·1 ± 11a

8·4 ± 2b

C20:5n- 3

15·6 ± 3a

476·6 ± 64b

10·0 ± 4a

13·2 ± 3a

400·9 ± 53b

3·7 ± 4a

C22:5n- 3

22·2 ± 4a

133·8 ± 20b

11·3 ± 5a

33·0 ± 4a

114·0 ± 17b

20·1 ± 6a

C22:6n- 3

387·6 ± 19a

1634 ± 180b

191·3 ± 32a

439·3 ± 19a

1556 ± 150b

73·4 ± 34a

PUFA

3219 ± 476a

3144 ± 539a

6404 ± 451b

2844 ± 476a

2962 ± 451a

5999 ± 476b

Δ9 Desaturase indexf

58·8 ± 2a, A

34·4 ± 3b

44·5 ± 2c

27·0 ± 2a, B

34·2 ± 2a, b

39·3 ± 2b

n-6/n-3 ratio

5·3 ± 0·3a

0·3 ± 0·02b

37·6 ± 6c, A

4·0 ± 0·3a

0·4 ± 0·02b

52·3 ± 6c, B

  1. DU6-mouse line selected for high body weight; DUhTP-mouse line selected for high treadmill performance n-3 and n-6 HFD -high fat diet enriched with either n-3 or n-6 polyunsaturated fatty acids
  2. LSM-Least square means, SE-standard error, fatty acid concentrations in mg/100 g fresh tissue;
  3. MUFA-monounsaturated fatty acids; SFA-saturated fatty acids; PUFA-polyunsaturated fatty acids; FA-fatty acids;
  4. a, b, c-significant effect of diet at p ≤ 0.05 within the selected line
  5. A, B-significant effect of selection line at p ≤ 0.05 within the feeding group
  6. + Sum of saturated fatty acids (C10:0+C11:0+C12:0+C13:0+C14:0+C15:0+C16:0+C17:0+C18:0+C20:0+C21:0+C22:0+C23:0+ C24:0)
  7. # Sum of MUFA (C14:1+C16:1+C17:1+∑ C18:1 trans isomers+C18:1cis- 9+C18:1cis- 11+C22:1+C24:1)
  8. d Sum of n-6 fatty acids (C18:3n-6+ C22:4n-6+ C20:3n-6+ C18:2n-6+ C20:4n-6)
  9. e Sum of n-3 fatty acids (C20:3n-3+ C22:6n-3+ C22:5n-3+ C20:5n-3+ C18:4n-3+ C18:3n-3)
  10. f Δ9 Desaturase index = ((C14:1+C16:1+C18:1+C17:1)/(C14:1+C16:1+C18:1+C17:1+C14:0+C16:0+C17:0+ C18:0))*100.