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Table 5 Effect of diet on fatty acid composition of quadriceps femoris muscle of different mice line

From: Metabolic responses to high-fat diets rich in n-3 or n-6 long-chain polyunsaturated fatty acids in mice selected for either high body weight or leanness explain different health outcomes

  DU6 DUhTP
  Standard chow n-3 HFD n-6 HFD Standard chow n-3 HFD n-6 HFD
  LSM ± SE LSM ± SE LSM ± SE LSM ± SE LSM ± SE LSM ± SE
SFA+ 365·5 ± 45a 697·2 ± 48b, A 376·6 ± 43a 327·0 ± 45 325·1 ± 43B 218·2 ± 45
C14:0 11·21 ± 4a 62·31 ± 4b, A 9·58 ± 4a 6·5 ± 4a 24·1 ± 4b, B 3·9 ± 4a
C16:0 265·9 ± 34a 488·5 ± 36b, A 248·4 ± 32a 199·3 ± 34 210·0 ± 32B 120·2 ± 34
C18:0 62·4 ± 6a 98·4 ± 6bA 91·2 ± 6a, b 85·2 ± 6a 57·7 ± 6b B 68·9 ± 6a, b
MUFA# 225·6 ± 50a 568·2 ± 53b, A 375·7 ± 47 180·0 ± 50 280·7 ± 47B 183·1 ± 50
C16:1cis-9 70·4 ± 10a, b, A 96·6 ± 10a, A 36·7 ± 9b 25·4 ± 10B 41·6 ± 9B 14·1 ± 10
C18:1cis- 9 206·7 ± 33 320·8 ± 35A 296·4 ± 31A 116·8 ± 33 149·5 ± 31B 145·8 ± 33B
n-6 FAd 345·0 ± 44a 141·6 ± 47b 800·6 ± 42cA 302·5 ± 44a 70·6 ± 42b 445·0 ± 44a, B
C18:2n-6 225·6 ± 42a 100·9 ± 44a 653·3 ± 40b A 167·5 ± 42a, B 43·2 ± 40a 318·7 ± 42bB
C20:4n-6 98·2 ± 5a 25·4 ± 5b 112·7 ± 5a, A 108·4 ± 5a 20·4 ± 5b 91·2 ± 5aB
n-3 FAe 99·5 ± 12a, A 325·0 ± 13b, A 37·9 ± 12c 171·8 ± 12a, B 270·6 ± 12b, B 25·7 ± 12c
C18:3n- 3 9·4 ± 1·6a 48·0 ± 5b, A 2·3 ± 0·3a 5·7 ± 1·6 6·8 ± 5B 1·3 ± 0·4
C20:5n- 3 0·9 ± 0·1a 50·5 ± 0·5b, A 1·0 ± 0·2a, A 1·3 ± 0·1a 26·4 ± 4bB 0·1 ± 0·2c, B
C22:5n- 3 7·9 ± 1a, A 23·1 ± 1b 3·1 ± 1a 22·3 ± 1a, B 20·5 ± 1a 2·7 ± 1b
C22:6n- 3 80·6 ± 9a, A 201·2 ± 9b 30·7 ± 8c 142·1 ± 8a, B 215·6 ± 8b 21·3 ± 9c
PUFA 445·2 ± 50a 468·8 ± 53a 839·6 ± 48b, A 475·0 ± 50 342·0 ± 47 471·7 ± 50B
Δ9 Desaturase indexf 43·4 ± 2a, b 41·2 ± 2a 48·5 ± 2b, A 33·7 ± 2a 42·5 ± 2b 45·0 ± 2b, B
n-6/n-3 ratio 3·4 ± 0·2a, A 0·40 ± 0·02b, A 23·8 ± 2·6c 1·9 ± 0·2a, B 0·3 ± 0·02b, B 17·6 ± 2·8c
  1. DU6-mouse line selected for high body weight; DUhTP-mouse line selected for high treadmill performance
  2. n-3 and n-6 HFD-high fat diet enriched with either n-3 or n-6 polyunsaturated fatty acids
  3. LSM-Least square means, SE-Standard error, fatty acid concentrations in mg/100 g fresh tissue;
  4. MUFA-monounsaturated fatty acids; SFA-saturated fatty acids; PUFA-polyunsaturated fatty acids; FA-fatty acids;
  5. a, b, c-significant effect of diet at p ≤ 0.05 within the selected line,
  6. A, B -significant effect of selection line at p ≤ 0.05 within the feeding group; all interaction DxL were significant at p ≤ 0.05;
  7. + Sum of saturated fatty acids: (C10:0+C11:0+C12:0+C13:0+C14:0+C15:0+C16:0+C17:0+C18:0+C20:0+C21:0+C22:0+C23:0+ C24:0)
  8. # Sum of MUFA (C14:1+C16:1+C17:1+∑ C18:1 trans isomers+C18:1cis- 9+C18:1cis- 11+C22:1+C24:1)
  9. d Sum of n-6 FA (C18:3n-6+ C22:4n-6+ C20:3n-6+ C18:2n-6+ C20:4n-6)
  10. e Sum of n-3 FA (C20:3n-3+ C22:6n-3+ C22:5n-3+ C20:5n-3+ C18:4n-3+ C18:3n-3)
  11. f Δ9 Desaturase index = ((C14:1+C16:1+C18:1+C17:1)/(C14:1+C16:1+C18:1+C17:1+C14:0+C16:0+C17:0+ C18:0))*100.