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Table 5 Effect of diet on fatty acid composition of quadriceps femoris muscle of different mice line

From: Metabolic responses to high-fat diets rich in n-3 or n-6 long-chain polyunsaturated fatty acids in mice selected for either high body weight or leanness explain different health outcomes

 

DU6

DUhTP

 

Standard chow

n-3 HFD

n-6 HFD

Standard chow

n-3 HFD

n-6 HFD

 

LSM ± SE

LSM ± SE

LSM ± SE

LSM ± SE

LSM ± SE

LSM ± SE

SFA+

365·5 ± 45a

697·2 ± 48b, A

376·6 ± 43a

327·0 ± 45

325·1 ± 43B

218·2 ± 45

C14:0

11·21 ± 4a

62·31 ± 4b, A

9·58 ± 4a

6·5 ± 4a

24·1 ± 4b, B

3·9 ± 4a

C16:0

265·9 ± 34a

488·5 ± 36b, A

248·4 ± 32a

199·3 ± 34

210·0 ± 32B

120·2 ± 34

C18:0

62·4 ± 6a

98·4 ± 6bA

91·2 ± 6a, b

85·2 ± 6a

57·7 ± 6b B

68·9 ± 6a, b

MUFA#

225·6 ± 50a

568·2 ± 53b, A

375·7 ± 47

180·0 ± 50

280·7 ± 47B

183·1 ± 50

C16:1cis-9

70·4 ± 10a, b, A

96·6 ± 10a, A

36·7 ± 9b

25·4 ± 10B

41·6 ± 9B

14·1 ± 10

C18:1cis- 9

206·7 ± 33

320·8 ± 35A

296·4 ± 31A

116·8 ± 33

149·5 ± 31B

145·8 ± 33B

n-6 FAd

345·0 ± 44a

141·6 ± 47b

800·6 ± 42cA

302·5 ± 44a

70·6 ± 42b

445·0 ± 44a, B

C18:2n-6

225·6 ± 42a

100·9 ± 44a

653·3 ± 40b A

167·5 ± 42a, B

43·2 ± 40a

318·7 ± 42bB

C20:4n-6

98·2 ± 5a

25·4 ± 5b

112·7 ± 5a, A

108·4 ± 5a

20·4 ± 5b

91·2 ± 5aB

n-3 FAe

99·5 ± 12a, A

325·0 ± 13b, A

37·9 ± 12c

171·8 ± 12a, B

270·6 ± 12b, B

25·7 ± 12c

C18:3n- 3

9·4 ± 1·6a

48·0 ± 5b, A

2·3 ± 0·3a

5·7 ± 1·6

6·8 ± 5B

1·3 ± 0·4

C20:5n- 3

0·9 ± 0·1a

50·5 ± 0·5b, A

1·0 ± 0·2a, A

1·3 ± 0·1a

26·4 ± 4bB

0·1 ± 0·2c, B

C22:5n- 3

7·9 ± 1a, A

23·1 ± 1b

3·1 ± 1a

22·3 ± 1a, B

20·5 ± 1a

2·7 ± 1b

C22:6n- 3

80·6 ± 9a, A

201·2 ± 9b

30·7 ± 8c

142·1 ± 8a, B

215·6 ± 8b

21·3 ± 9c

PUFA

445·2 ± 50a

468·8 ± 53a

839·6 ± 48b, A

475·0 ± 50

342·0 ± 47

471·7 ± 50B

Δ9 Desaturase

indexf

43·4 ± 2a, b

41·2 ± 2a

48·5 ± 2b, A

33·7 ± 2a

42·5 ± 2b

45·0 ± 2b, B

n-6/n-3 ratio

3·4 ± 0·2a, A

0·40 ± 0·02b, A

23·8 ± 2·6c

1·9 ± 0·2a, B

0·3 ± 0·02b, B

17·6 ± 2·8c

  1. DU6-mouse line selected for high body weight; DUhTP-mouse line selected for high treadmill performance
  2. n-3 and n-6 HFD-high fat diet enriched with either n-3 or n-6 polyunsaturated fatty acids
  3. LSM-Least square means, SE-Standard error, fatty acid concentrations in mg/100 g fresh tissue;
  4. MUFA-monounsaturated fatty acids; SFA-saturated fatty acids; PUFA-polyunsaturated fatty acids; FA-fatty acids;
  5. a, b, c-significant effect of diet at p ≤ 0.05 within the selected line,
  6. A, B -significant effect of selection line at p ≤ 0.05 within the feeding group; all interaction DxL were significant at p ≤ 0.05;
  7. + Sum of saturated fatty acids: (C10:0+C11:0+C12:0+C13:0+C14:0+C15:0+C16:0+C17:0+C18:0+C20:0+C21:0+C22:0+C23:0+ C24:0)
  8. # Sum of MUFA (C14:1+C16:1+C17:1+∑ C18:1 trans isomers+C18:1cis- 9+C18:1cis- 11+C22:1+C24:1)
  9. d Sum of n-6 FA (C18:3n-6+ C22:4n-6+ C20:3n-6+ C18:2n-6+ C20:4n-6)
  10. e Sum of n-3 FA (C20:3n-3+ C22:6n-3+ C22:5n-3+ C20:5n-3+ C18:4n-3+ C18:3n-3)
  11. f Δ9 Desaturase index = ((C14:1+C16:1+C18:1+C17:1)/(C14:1+C16:1+C18:1+C17:1+C14:0+C16:0+C17:0+ C18:0))*100.