Figure 3

Human serum affects SCD1 expression and cholesterol efflux in THP-1 MDFC. Human serum affects SCD1 expression in THP-1 MDFCs. Samples were taken from 15 subjects consuming different walnut components: whole walnut, walnut oil, defatted walnut meat, and walnut skin. Baseline serum as well as postprandial serum of 1, 2, 4, 6 h were applied as treatment (10%, v:v) for 24 h in serum free media. Real time PCR value of individual serum treated sample is divided by a PCR value of a pooled serum treated sample to minimize the variation between PCR runs and to exclude a non-specific serum effect on gene expression. Value of each postprandial serum treated sample is adjusted by baseline value, which served as a control within the same dietary period. Data were logarithm transformed for analysis. Sera of fifteen participants at baseline and 1, 2, 4, and 6 h postprandially were applied as treatment. N = 75 of each dietary group.