Leucine supplementation increases adiponectin levels. (A) Adiponectin. *, effect of diet (P < 0.021). (B) TNF-α. #, effect of training (P < 0.044). (C) Leptin. Experimental groups: control diet (REF), diet supplemented with 5% L-leucine (LS), 6 weeks of endurance training (ET) plus standard diet, and diet supplemented with 5% L-leucine plus endurance training (LS+ET). Values are mean ± sem (n = 6-8 rats per group).