Effect of high fat diet with different trans fatty acid levels on rat's liver histology. Normal liver histological aspect from a control (H&E 32 ×). Panel (a) it is composed of hexagonal or pentagonal lobules with central veins (CV) and peripheral hepatic triads (HT) embedded in connective tissue. Hepatocytes are arranged in trabecules running radiantly from the central vein and are separated by sinusoids (S) containing Kuppfer cells. Liver from experimental groups (H&E 100×): FSO Panel (b): moderate lipid accumulation is seen in many hepatocytes; OSO Panel (b); abundance of cytoplasm vacuolization and ballooned hepatocytes and MG Panel (e); severe lipid accumulation in hepatocytes and high number of ballooned hepatocytes. Cytoplasm vacuolization in parenchymatous cells of the liver (thin arrow), hypertrophied hepatocytes (black triangle). Liver from OSO Panel (d) and MG Panel (f) groups (H&E 32×): photomicrograph of degenerated hepatocytes and necrosis (thick arrow).