Figure 2From: UPR in palmitate-treated pancreatic beta-cells is not affected by altering oxidation of the fatty acidUnfolded protein response in palmitate-exposed human islets. The islets were cultured for 3, 24 or 48 hours in the presence of 0.5 mM palmitate (Pa) at 5.5 or 25 mM glucose (Glu). AICAR (1 mM, Aic) or etomoxir (0.2 mM, Et) were added as indicated. Islets cultured at 5.5 mM glucose alone were considered as control. After culture, protein levels of p-eIF2α (A), CHOP (B) and BiP (C) were measured. Protein levels are means ± SEM of 4 independent experiments.*P < 0.05 compared to control.Back to article page