Table 1 Primers used for real-time PCR
Target | Forward primer | Reverse primer |
|---|---|---|
b-actin | 5'-TCTGTGTGGATTGGTGGCTC-3' | 5'-GACTCATCGTACTCCTGCTTGCT-3' |
CHOP/GADD153 | 5'-CCAGCAGAGGTCACAAGCAC-3' | 5'-CGCACTGACCACTCTGTTTC-3' |
GADD34 | 5'-GTCCATTTCCTTGCTGTCTG-3' | 5'-AAGGCGTGCCCATGCTCTGG-3' |
ATF4 | 5'-GTTGGTCAGTGCCTCAGACA-3' | 5'-CATTCGAAACAGAGCATCGA-3' |
Spliced XBP1 rat | 5'-GAGTCCGCAGCAGGTG-3' | 5'-GCGTCAGAATCCATGGGA -3' |
EDEM | 5' -CAGTCAAGTTAGTGATCAACAC- 3' | 5' - TGTCATGTCACCAAAGGGCTG - 3' |