Assessment of body weight, plasma TG, and hepatic steatosis in zebrafish overfed with Campari and regular tomatoes. (A) Feeding condition of each group. Group 1, normal feeding; group 2, overfeeding of Artemia with Tetramin (vehicle); group 3, overfeeding with regular tomato; group 4, overfeeding with Campari tomato. Feeding experiments were conducted over 2 and 4 weeks. (B) Average body weight in each group during 4-week feeding experiments. Values are mean ± SD. Each group contained 20 samples. **P < 0.05; ***P < 0.01 vs. vehicle with overfeeding, black circles. (C) Change of plasma TG levels in the each group. ***P < 0.01 vs. vehicle with overfeeding, black circles. (D) Feeding behaviour assay counting Artemia numbers 90 min after feeding at 2 weeks. No significant difference was observed among vehicle, regular tomato, and Campari tomato administration in overfeeding groups. (E) Oil red O staining of liver sections. Campari tomato reduced the number and size of lipid droplets (red) compared with overfeeding and overfeeding regular tomato. (F and G) qPCR-assessed gene expression in the livers of DIO and normally fed zebrafish. Expression of ppar-ab (F) and ppargc1-like (G), a zebrafish homolog of human PPAR-α and PPARGC1 (PGC-1α, was normalized to actb expression. Values are mean ± SE (n = 5/group). *P < 0.05 vs. vehicle with overfeeding (OF).