Figure 6
From: Effects of dairy consumption on SIRT1 and mitochondrial biogenesis in adipocytes and muscle cells
Effects of serum treatment on mitochondrial biogenesis in mouse skeletal muscle cells. C2C12 muscle cells were grown in co-culture with 3T3-L1 adipocytes and incubated for 48 hours with human serum collected on day 0 (baseline), 7 or 28 after high dairy diet or soy-based control diet. (a) Cytochrome c oxidase subunit 7 (COX 7), (b) nuclear respiratory factor 1 (NRF1) and (c) UCP 3 were significantly up-regulated 28 days after dairy feeding compared to baseline and soy-based control. Values are expressed as means of % change to baseline ± SE (n = 6 subjects/group). Gene expression data are normalized to 18S. Differing letters above the bars denote significant differences between groups, p ≤ 0.05.